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How to fast identify liquid reagent on quanitification method?
According to the traditional method, Raman spectroscopy is considered to be unsuitable for quantitative applications due to poor signal strength stability, strong background signals such as fluorescence, and the like, even in many textbooks. But in fact, material information contained in Raman spectroscopy Not only the nature of the material, but also the information about the quality of the material. In principle, as long as the current difficult problem is solved in the method, Raman spectroscopy is a simple, fast and non-destructive testing method, not only a powerful tool on qualification, but also on quantification.
In order to solve this problem, Sipatt proposed a multi-technology centered on "high-order derivatives to ensure data selectivity" and successfully applied to Raman spectrometers. In this paper, we will introduce a salicylic acid saponin as an example to introduce a method for the detection of salicylic acid and benzoic acid in salicylic acid bismuth, and the accuracy is expressed as 3% within the relative error. Optosky Raman spectrometers make a fast, viable, no-module method for quantitative analysis of liquid formulations.
Set up the mothod:
- Solution preparation: 9 sets of standard solutions of known concentrations of barium salicylate were prepared in 75% ethanol solution for establishing the working curve.
- The build of the working curve: The spectra were acquired using a handheld Raman spectrometer and a software calibration module was used to set up a working curve.
Verify Method:
3 sets of known concentrations of barium salicylate solution prepared in 75% ethanol solution were used for method accuracy verification. Spectra were acquired using a handheld Raman spectrometer and the content was calculated using a software quantitative module. The results show that the relative error between the measured value of salicylic acid and the known concentration is within 3%, and the relative error between the measured value of benzoic acid and the known concentration is within 2%.
Summar:
The significant resolution advantage of the handheld Raman allows the measured values to directly reflect the content of each component. The quantitative method adopts the method of improving internal standard and angle measurement to make full use of the accurate relative response relationship of each component on the spectrum to effectively solve the instability of signal intensity.
In handheld Raman spectrometer, quantitative technology based on "high-order derivative to ensure data selectivity", the relative error of salicylic acid in the test salicylic acid bismuth agent is within 3%, and the relative error of benzoic acid test results is within 2%. This method does not require complex sample pretreatment and is not affected by water. It is particularly noteworthy that the new method does not follow the multivariate statistical analysis approach. The quantitatively corrected samples are significantly less than the number of samples required for multivariate analysis such as PLS. The traditional single factor quantitative method is simple and fast.
Specific technical details are welcome. Optosky looks forward to being your partner and friend in new areas and new technologies.
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